Coding
FcPT1

Part:BBa_K4179002:Design

Designed by: Yasmin Habib   Group: iGEM22_Technion-Israel   (2022-09-18)


Ficus caricia prenyl transferase (FcPT1)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 862
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1279
    Illegal SpeI site found at 862
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 862
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 862
    Illegal NgoMIV site found at 56
    Illegal NgoMIV site found at 621
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 137
    Illegal SapI site found at 933


Design Notes

This sequence was codon optimized for E. coli.

Source

Ficus carica [1]

References

1. Munakata R, Kitajima S, Nuttens A et al. Convergent evolution of the UbiA prenyltransferase family underlies the independent acquisition of furanocoumarins in plants. New Phytologist 2020; 225: 2166–2182.